Quality & quantification
Short-read RNA-seq quality control and Salmon transcript quantification.
AVAILABLE NOW / RESEARCH SERVICE
One gene.
More to discover.
Human differential transcript usage analysis for researchers looking beyond changes in total gene expression.
Request a project assessment ↗THE QUESTION WE ASK
Which transcripts
are gaining ground?
DTU examines how the relative use of transcripts within a gene differs between conditions. It complements differential expression analysis by asking about composition as well as abundance.
MicroTarget connects these findings with expression context, transcript structure, and selected functional annotations to support research interpretation.
TRANSCRIPT RESOLUTIONILLUSTRATIVE MODEL
THE ANALYTICAL WORKFLOW
From sequencing reads
to prioritized candidates.
Statistical assessment
DRIMSeq differential transcript usage testing with stageR stage-wise statistical error control.
Expression & evidence
Transcript and gene-level expression context, with HISAT2 and RegTools splice-junction evidence where applicable.
Interpretation & reporting
Transcript structure, selected protein consequence and functional annotations, and research-oriented candidate prioritization.
Methods and supporting analyses are scoped to the study. Junction evidence and computational annotations do not constitute experimental biological validation.
STUDY ELIGIBILITY
A precise service starts
with the right study.
We review experimental design and data suitability before defining analytical scope.
- Human bulk RNA-seq
- Paired-end sequencing
- Read lengths of at least 100 bp
- Appropriate two-group comparison design
- Suitable quality, replication, and study metadata
Eligibility is project-specific. Share your design for review; no universal minimum sample count or guaranteed outcome is implied.
REPRESENTATIVE DELIVERABLES
Results with context.
Research-ready tables
Transcript-level candidates, statistical summaries, and expression context organized for investigation.
Figures & evidence
Visual summaries of usage shifts and applicable structural or splice-junction supporting evidence.
Interpretive priorities
Selected functional annotations and candidate prioritization to guide subsequent research questions.
The exact deliverable package, optional analyses, and reporting scope are agreed in the project quote.
FOUNDING CLIENT PROGRAM
Built around your research question.
Eligible early clients may receive preferential launch pricing. Quotes reflect sample count, design, data quality, analytical scope, and reporting requirements.
For research use only. Not for diagnostic or clinical decision-making.
START A CONVERSATION
Let’s assess your study’s fit.
Tell us about your research question. We’ll help assess whether transcript usage analysis fits your study.